FastQCFastQC Report
Thu 28 Apr 2016
ERR1042140.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042140.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2639208
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT253500.9605154273554795No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT180410.6835762850067141No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127600.4834783768463873No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT70630.26761816423714996No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT30460.1154134119023586No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT29690.11249586997311314No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27100.1026823198474694No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26910.10196240690388936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC7900.018.9683538
GACGGAC8000.018.96257
GTATACT2950.018.1864414
CGGACCA8150.018.1595129
CAAGACG9050.017.784534
GCGCAAG9350.017.4117661
CGCAAGA9100.017.280222
CGAAAGC9050.016.7624319
GCGAAAG9150.016.37704818
AGACGGA9450.016.2486786
ACCGTCG2400.016.18758
TACCGTC2200.015.9772737
AAGACGG10500.015.8571425
AGCGAAA9500.015.773684517
GCTAGAC1902.7284841E-1115.5789483
TATAGTG2850.015.5789475
TACGACC1101.4532079E-515.1363644
GGTATCA84000.015.0863091
TCGTATA750.004106280414.82
GTAAGAC3000.014.83
Produced by FastQC (version 0.11.5)