FastQCFastQC Report
Thu 28 Apr 2016
ERR1042139.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042139.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11571563
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT704580.6088892226573022No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT464050.40102620536223155No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT410250.354532918327455No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300780.2599303136490723No Hit
GTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA131740.11384806011080785No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA4900.014.34693816
TATACTG21300.013.37558655
ACGGACC11200.013.0491088
AACGCAG360700.012.7863885
GGACCGT6600.012.3333346
TATACCG4050.012.3333335
GTATTAG25500.012.0431381
TCGTTTA6300.012.03968330
ACGAACG5850.012.01709515
GGTATCA280700.012.0016021
TCTATAC16150.011.6842113
GTACATG350150.011.6447241
TACATGG351050.011.5410912
CTTATAC59950.011.38698937
GTGTAAG21850.011.0915331
TATACAG24050.011.0769235
TCTTATA85350.011.07615737
ACATGGG360750.011.07179453
CTACACT25650.011.0350884
TAACGCC6900.010.9927534
Produced by FastQC (version 0.11.5)