FastQCFastQC Report
Thu 28 Apr 2016
ERR1042138.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042138.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2454814
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT322091.3120749677979675No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT232410.9467519738766359No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155470.6333270056305691No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT79310.323079467527886No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT38220.15569407702579502No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32820.13369648372544723No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29730.12110897200358153No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAG5250.016.2095245
CGTCTAA700.002593617715.8571431
TAACGCT957.0648966E-515.5789484
TAGACTC1557.219569E-915.51612955
GCAATAC2900.015.3103463
CTATACT3800.015.0921064
TAACACT4550.015.0439564
GTAACAC3100.014.9193553
CTAGACT2251.8189894E-1214.84
TCTATAC3200.014.4531263
TATACTG4000.014.3375015
ACGGACC3250.014.2307698
TATACCG1304.448626E-614.2307695
TATAGTG4200.014.0952385
GTAATCC11200.014.0401794
GGTATCA112800.013.7765951
CTACCCG950.001245778513.63157910
TCCGACC3000.013.5666688
AACGCAG142950.013.5498435
ACTCCGC1102.458142E-413.4545458
Produced by FastQC (version 0.11.5)