FastQCFastQC Report
Thu 28 Apr 2016
ERR1042137.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042137.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9893034
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT639990.6469097346678481No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT445020.44983166943528147No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT396350.4006354370155809No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263280.2661266503278974No Hit
GTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA112300.11351421616462654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC12250.014.1959188
GACGGAC12300.013.83739957
TATACTG20100.013.8059695
CTATACT21550.012.619494
GGTATCA278100.012.3000721
TCTATAC14000.012.2892863
TCTAGCG6150.012.03252128
TCTAGAC10900.011.88073353
GTACATG351650.011.8580971
AAGACGG16250.011.7261535
TACATGG354450.011.7017922
TACCGTC7300.011.6575357
CGCGTAT1757.3478495E-611.62857112
AACGCAG341450.011.6271785
GTATTAG23750.011.5284211
TAGACTG14500.011.48275855
GTATAGG12250.011.4775511
CGGGTAA5000.011.46999924
CATGGGA227850.011.4077244
ACATGGG359100.011.36483
Produced by FastQC (version 0.11.5)