FastQCFastQC Report
Thu 28 Apr 2016
ERR1042136.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042136.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1770410
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT536053.027829711761682No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT336281.8994470207466068No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232391.3126337966911619No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT106930.603984387797177No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT59130.3339904315949413No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48400.2733830016775775No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39140.2210787331748013No Hit
GAGTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAA36840.20808739218599082No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG35790.20215656260414255No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAA32940.18605859659626867No Hit
GTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA30930.1747052942538734No Hit
GAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAAAAAAAAAA30400.17171163741732143No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26230.14815777136369543No Hit
CATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAAAAA24300.13725634175134574No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA22090.12477335758383651No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACAAAAAA21070.11901198027575532No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18930.106924384747036No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACAAAA18440.1041566642755068No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT18350.10364830745420552No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA17830.10071113470890923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACAC1153.0540832E-919.3043483
CGTATAC701.2196697E-418.53
TCGCGTA1258.587449E-917.7600029
CGCAAGA4000.017.11252
CGCGTAA1301.3944373E-817.07692110
CGGACCA3950.016.860769
GTATACT1655.4569682E-1116.8181824
ACGGACC3900.016.6025648
GTCGCGT1352.2215318E-816.4444438
GACGGAC4100.016.2439027
TACACCG1151.2430464E-616.0869565
GCGTAAC1504.6748028E-916.03333311
TTTAGAC1403.4762706E-815.8571423
CTAGTAC700.002593235815.8571423
CAAGACG5200.015.6538454
AAGACGG4700.015.3510655
GCGCAAG4700.015.3510651
ACAGCGT855.3661846E-415.2352948
GCGAAAG4450.014.96629318
TACACTG4350.014.8850585
Produced by FastQC (version 0.11.5)