FastQCFastQC Report
Thu 28 Apr 2016
ERR1042135.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042135.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9187292
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT487750.530896372946457No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT370300.4030567440329534No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT243180.26469170676190545No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211070.22974125563876713No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA154100.016.4471131
TATACTG16850.014.3827895
TACCGTC6250.012.7280017
ATACCGT9400.012.5957456
TAGACCG1501.946919E-512.3333335
ACGGACC8850.012.3333338
GTATCAA209350.012.1330312
TAGACAG17450.012.085965
AACGCAG259100.012.0096495
TATAGTG13700.011.8832115
TACACAG27250.011.8807345
TTATACT21500.011.8744194
CGTCGTA5150.011.85436810
TAGGGTG16900.011.7130175
ATCAACG265850.011.6212162
CTACACT19750.011.521524
TCAACGC271550.011.4454063
TCTATAC12200.011.3729513
ACACCGT6850.011.3430656
GACGGAC8650.011.3352597
Produced by FastQC (version 0.11.5)