FastQCFastQC Report
Thu 28 Apr 2016
ERR1042134.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042134.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7273828
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT743151.0216766192436775No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT677570.9315177647862997No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT387280.5324294168077661No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT348080.4785375733382753No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC90520.1244461650729162No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT86380.11875452650241386No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG85310.11728349914240478No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA83300.11452016737266814No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG81100.1114956251371355No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC78850.10840234330534074No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT78810.10834735162833105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG20700.018.8574895
CAAGACG21300.018.326294
GACGGAC20450.018.1833747
ACGGACC21000.017.7071448
GCGCAAG21700.017.6474651
CGCAAGA22400.017.5915162
CGGACCA21300.017.2840379
CGCGTAT650.001581028717.07692314
TATAGCG1151.244347E-616.0869562
AGACGGA23750.015.9684216
CAAATCG18650.015.37533613
GCAAGAC30100.015.1810643
TCTAGCG8700.014.88505828
CTAGCGG9000.014.829
AAATCGC19450.014.7429314
ATCGCTC19550.014.57288916
TATCTAG12650.014.4782611
TATACTG12150.014.4650215
TTCGGGC12000.014.18333335
CGAAAGC27300.013.82417619
Produced by FastQC (version 0.11.5)