FastQCFastQC Report
Thu 28 Apr 2016
ERR1042133.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042133.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4777886
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT982912.0572068902439278No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT792831.6593740411554396No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT458070.9587294464539339No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430110.9002098417584681No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA94910.19864433768407203No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA94730.19826760203152607No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT89970.18830503699753406No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87290.18269586172629484No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85440.17882385640846182No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80460.16840083668802477No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55350.1158462131578694No Hit
GTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA52990.11090679015782294No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC49830.10429298647979462No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG11800.020.0677975
GCGCAAG12550.019.4581661
ACGGACC11800.019.2838978
GACGGAC12000.019.1166677
CAAGACG13400.018.638064
CGCAAGA13800.018.0978262
CGGACCA12650.017.8418969
AGACGGA13750.017.2218176
CAAATCG10300.016.16504913
TCTAGCG3350.016.01492528
CTATCGA700.002594100515.8571429
GGTATCA359650.015.5911311
TATACTG7200.015.4166665
CTTACAC6150.015.3414633
TACCGTC8900.015.1741567
GCGAAAG15150.015.14191218
AAATCGC11000.015.13636314
GCAAGAC19050.015.0524953
CGTCGTA9000.015.00555610
CGAAAGC15250.014.819
Produced by FastQC (version 0.11.5)