FastQCFastQC Report
Thu 28 Apr 2016
ERR1042132.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042132.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1071959
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT163621.5263643478901712No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT146701.3685224901325517No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73660.6871531467154993No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT42260.3942314957941488No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT20850.19450370769777575No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18650.17398053470328623No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15550.14506151821105098No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15420.1438487852613766No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14390.1342402088139565No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC14020.13078858426488327No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12140.11325060006959221No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG11840.11045198557034365No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA11160.10410845937204687No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACAT809.786163E-1025.437514
TATACCG400.00193110323.1255
GACGGAC4600.021.3152187
CGGACCA4600.021.3152189
AAGACGG4800.021.1979185
GACGTCG1059.822543E-1021.14285737
ACGGACC4650.021.0860218
GCGACGT1001.2894816E-820.34999835
GCCGACA1001.2894816E-820.34999813
GGTATCA55300.020.139241
AGACGGA5150.020.1165056
CAAGACG5150.019.757284
AAGCGAC951.6758531E-719.47368433
CGACATC1052.259003E-819.3809515
CGCAAGA5150.019.0388342
ACACGCT3800.018.59
CGACGTC1103.8508006E-818.536
TAGGTTG801.6165051E-518.55
CGTGAAA902.1519409E-618.510
TCGCGGG701.2191001E-418.533
Produced by FastQC (version 0.11.5)