FastQCFastQC Report
Thu 28 Apr 2016
ERR1042131.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042131.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3107041
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT327771.0549265362124285No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT297630.9579210573661564No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT200460.6451797707207597No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130080.41866200027614703No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC38000.12230285985926803No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG33640.10827021593857306No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA33540.10794836630736446No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105000.022.1295241
TACGACG4200.017.1785725
CAACGCG700.002593826715.8571424
ACGGACC3900.015.6538478
GACGGAC4150.015.6024097
GTATCAA150550.015.5938232
CGTCGTG3950.015.45569630
ATCGCTC5450.015.27522916
CGAACGA5100.015.23529416
GACGGTA4700.014.9574478
TAGACCG4100.014.890244525
TATACCG1252.9624898E-614.7999995
AAGACGG4150.014.7108435
GTCGTGA3900.014.70512931
CAAATCG5700.014.60526413
AAATCGC5850.014.54700814
ATAACGC5650.014.4070793
GCGGGTA5150.014.36893323
ACGGTAT4900.014.346949
GCGCAAG4550.014.2307691
Produced by FastQC (version 0.11.5)