FastQCFastQC Report
Thu 28 Apr 2016
ERR1042130.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042130.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8731092
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT664600.7611877185579994No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT586270.6714738545877194No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT397450.4552122460741452No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257620.2950604575006196No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT93100.10663041919613263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG21000.020.0857145
GACGGAC19800.019.6212127
ACGGACC21000.019.0285728
CGGACCA22300.018.1681619
CGCAAGA22600.018.0907062
GCGCAAG24150.016.9296051
AGACGGA24850.016.378276
CAAGACG27050.016.2772644
TCTAGCG11550.015.6969728
CTAGCGG11400.015.57894829
GGTATCA271200.015.4575971
GTATTAG28950.015.2728841
GTAAACG15350.015.18566727
GCGAAAG25800.014.77131818
CGAAAGC26200.014.68702319
CGAACGA11600.014.67241516
TAAACGC15900.014.66037828
CGAACTA7200.014.64583329
CGGTCCA13700.014.44890510
ACGCTTC16500.014.35151631
Produced by FastQC (version 0.11.5)