FastQCFastQC Report
Thu 28 Apr 2016
ERR1042129.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042129.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7924738
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT661900.8352326600576575No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT599660.7566937859649114No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT387810.48936633614890485No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254150.3207046087832809No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT99290.12529120836550053No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA81630.10300655996450607No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC80700.10183301959004827No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC22600.019.2367278
AAGACGG24550.018.9898175
GACGGAC22750.018.9472527
CGGACCA24450.018.008189
CAAGACG25400.017.553154
TCTAGCG11700.016.91880428
CGCAAGA26450.016.7164462
GTAAACG14050.016.59074827
CTAGCGG11900.016.3235329
AGACGGA27150.016.285456
GCGCAAG27450.016.1074681
GGTATCA271050.016.0667761
TCGTTTA14250.015.18947430
CGCTTCG15750.015.15238132
TATACTG13850.015.0938635
TTCGGGC15450.015.08737835
GTATTAG20400.015.0539221
TAAACGC15300.014.993463528
CGAGCCG21650.014.61200815
GCGAAAG29150.014.59691218
Produced by FastQC (version 0.11.5)