FastQCFastQC Report
Thu 28 Apr 2016
ERR1042128.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042128.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3960438
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT412251.0409202214502538No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT393010.9923397361604953No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT174000.43934534513606827No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168750.426089235584549No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC62420.1576088301344447No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA54530.13768679120844715No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT47350.11955748328846456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130300.022.4612431
AAGACGG10400.020.1009625
ACGGACC10250.019.3121978
GACGGAC10550.018.9383897
CAAGACG11350.018.907494
CGGACCA10800.018.3287059
CGCAAGA11350.017.9295162
GCGCAAG11800.017.7161031
GTATCAA165900.017.63022
AGACGGA11700.017.0769216
CGAACGT2200.016.8181824
ACACGCT17800.015.3820229
TATACTG7700.015.1363635
GCAAGAC17000.015.0176483
CGGGTAA5950.014.92436924
GCGGGTA5850.014.86324723
TATATCG1752.242814E-914.7999995
TACCGTC8050.014.7080757
ACGCTGA19150.014.58746711
GACGGAA10950.014.52968135
Produced by FastQC (version 0.11.5)