FastQCFastQC Report
Thu 28 Apr 2016
ERR1042127.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042127.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2322911
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT237501.0224240188280997No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT230100.9905674388730348No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT150530.6480231054913426No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92430.3979059034117106No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA45470.1957457689941629No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC44530.19169912235122225No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG33110.14253667058273No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC29900.1287178027914113No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA26990.11619041797124384No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC25820.11115363438375384No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC23260.10013297969659622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94800.018.870781
TTAGCGA500.007036991418.527
TCGTCGG500.007036991418.513
CTAGCGG2850.018.17543829
ACGGACC4950.017.9393948
AAGACGG5350.017.2897195
CGAACGT752.0684121E-417.2666664
TACTCCG1502.5102054E-1017.2666665
GACGGAC5300.017.1037737
CGCAATA3350.016.56716336
GTGTAAG4750.016.3578951
TCTAGCG3250.015.93846228
CAAGACG6650.015.8571434
ACACGCT11850.015.7679329
CGAACTA957.0646354E-515.57894829
CTTCGGG3700.015.534
AGACGGA6100.015.4672146
TAGACAG2900.015.3103465
CGGACCA5800.015.3103469
ACGAACG5100.015.23529415
Produced by FastQC (version 0.11.5)