FastQCFastQC Report
Thu 28 Apr 2016
ERR1042126.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042126.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4479602
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT523171.1678939334342648No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT435410.9719836717636968No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT251970.562483006302792No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162430.3625991773376296No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT61980.1383605061342503No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC61290.13682019072230078No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA60050.13405208766314508No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA58550.13070357589803738No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG50190.11204120366050377No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG45080.10063394024737018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC12800.020.0898448
AAGACGG13650.020.0586075
CAAGACG13550.019.933584
GACGGAC12950.019.8571437
TCGTTTA9700.019.26288630
CTAGCGG6750.018.63703729
TCTAGCG7000.018.23571428
CGGACCA14450.017.7958499
CCGGTCG10300.017.6019420
CGCAAGA14750.017.5593222
AGACGGA15000.017.5133326
CGGTCCA7100.017.45774710
CGTTTAT11050.016.90950231
GCGCAAG14750.016.6813561
ACACGCT19450.016.5501299
ATCGCCG11150.016.42600816
TAACGCC11550.016.3376624
TCGCCGG11500.015.92608717
CCGGTCC7800.015.8910259
TAGACAG7000.015.8571435
Produced by FastQC (version 0.11.5)