FastQCFastQC Report
Thu 28 Apr 2016
ERR1042125.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042125.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3057517
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT307431.0054890945823032No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT274700.8984414477499225No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT158770.5192775706561893No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102250.33442168923345317No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC46510.15211689746941717No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT45470.14871544459115027No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA45200.1478323750939079No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG38340.12539586860841656No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG36250.1185602565742071No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC34480.11277124542561826No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA33290.10887919838221667No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC32390.10593563339140878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG9450.021.3386235
CAAGACG10000.020.1649994
ACGGACC10100.019.2326728
GACGGAC10250.018.7707317
GCGCAAG10300.018.6796131
TCTAGCG3300.018.528
TTCGGGC5700.018.535
CGGACCA10500.018.3238099
CGCAAGA10850.017.9032272
TATACCG1156.415212E-817.6956525
AGACGGA11000.017.490916
GCTTCGG6300.017.32539733
CTAGCGG3700.016.529
CTTCGGG6400.016.47656234
TACACCG2150.016.3488375
GGTATCA129300.016.010441
GTAAACG6550.015.81679427
TCGTTTA6800.015.77941130
TAAACGC6700.015.73880628
CGCTTCG6700.015.73880632
Produced by FastQC (version 0.11.5)