FastQCFastQC Report
Thu 28 Apr 2016
ERR1042124.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042124.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3554773
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT364811.0262539970906723No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT320490.9015765563652025No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT188580.5304980092962336No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141470.3979719661424232No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC71000.19973145964594644No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT62260.17514479827544543No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG58060.1633296978456852No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC56530.1590256255462726No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA54450.15317433771439132No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG50040.1407684822631431No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG48080.13525476872925501No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA45250.12729364153491657No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC43760.12310209400150163No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA41010.11536601633915865No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG40960.11522536038166149No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA40410.1136781448491929No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC39270.110471189018258No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG37960.10678600293183278No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC36720.10329773518590356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG12000.021.5833345
CAAGACG12850.020.7315184
ACGGACC13200.019.0606068
CGCAAGA13400.019.0522382
GACGGAC13450.018.5687737
CTAGCGT500.007037707618.54
GCGCAAG14000.018.2357141
CGGACCA13800.018.2318849
AGACGGA14600.017.2328786
CTAGCGG4200.017.17857229
TCGTTTA8650.017.10982730
GCAAGAC17700.017.0367223
TCTAGCG4250.016.9764728
TATACTG6350.016.6062985
TAACGGC1901.8189894E-1216.55263136
TTAACGG1851.8189894E-1116.035
ACACGCT18050.015.8864279
TAATACT6450.015.7751944
CTTCGGG8750.015.64571534
GTATGGT13750.015.6072723
Produced by FastQC (version 0.11.5)