FastQCFastQC Report
Thu 28 Apr 2016
ERR1042123.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042123.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5419999
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT178760.32981555900656073No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT174660.3222509819651258No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT108550.2002767897189649No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC78690.14518452863183184No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT76470.14108858691671344No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA73370.13536902866587244No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG69790.12876386139554638No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC62860.11597788117673086No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA60680.1119557402132362No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC59470.10972326747661762No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58390.10773064718277622No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG57070.10529522237919231No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG55450.10230629193843026No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG16800.019.9315475
ACGGACC16350.019.6880748
GACGGAC16350.019.6880747
CAAGACG17450.019.4011464
TCTAGCG7400.019.028
CTAGCGG7450.018.87248229
CGGACCA18500.017.99
AGACGGA18750.017.4639996
GCGCAAG18700.017.2139051
CGCAAGA19950.016.5062662
TATACTG9750.016.317955
ATCGCTC13900.016.2374116
CCTATAC5100.015.5980393
TAGCGGC9350.015.43315630
CAAATCG14550.015.3848813
TCGTTTA10700.015.21495230
ACACGCT23850.015.2033559
AAATCGC14900.015.14765114
GTATGGT19300.015.1450783
AGTATGG20050.015.03992
Produced by FastQC (version 0.11.5)