FastQCFastQC Report
Thu 28 Apr 2016
ERR1042122.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042122.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8417708
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT665870.7910348042483772No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT604480.7181052134381474No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT412630.4901928173322239No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221510.26314764066418084No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA136760.16246702784178307No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC135420.16087514558594812No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT118960.14132112921949774No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG106690.12674471483211344No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG97410.11572033622453998No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC97160.11542334326636182No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA96510.11465116157509858No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC90020.10694122438079344No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG84330.10018166465265842No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG24550.020.798375
ACGGACC24800.019.9919348
GACGGAC25200.019.6746037
CAAGACG27200.019.4522064
TCTAGCG13100.019.20610828
CTAGCGG13350.018.98501829
CGCAAGA27300.018.635532
CGGACCA27350.018.1956129
GCGCAAG28500.017.9807011
GGTATCA282250.017.8019491
AGACGGA28650.017.5637026
CGCAATA15150.016.85148636
TCGTTTA16700.016.17365330
GCGAAAG31450.015.70588218
CGAAAGC31700.015.406939519
GCAAGAC37700.015.3594173
GAGCGAA32450.015.33590116
CGAGCCG23650.015.331923515
CGAACGA17500.015.32857116
TAACGAA17600.015.24147713
Produced by FastQC (version 0.11.5)