FastQCFastQC Report
Thu 28 Apr 2016
ERR1042121.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042121.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3050451
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT385751.264567108273498No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT344251.128521651388598No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT207430.6799978101598747No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC118420.38820489167011696No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106670.34968599725089833No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG103140.33811393790623095No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA71570.23462104456029617No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA66980.2195740892084482No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA64350.21095241326610395No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG64260.21065737492587164No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC61150.200462161168955No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT58200.19079146001689587No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG47370.1552885130756075No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA44800.1468635293600848No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG41950.13752064858606153No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC41560.13624214911172153No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC40570.13299672736916607No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG38750.12703039648891262No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT37540.12306376991467818No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG36900.12096571949524841No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG35900.11768751571488936No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT35290.11568781140887036No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC34660.11362254302724416No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC32050.1050664311605071No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC30930.10139484292650497No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT30790.1009358943972547No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAG400.001931915823.1251
AAGACGG10500.022.023815
ACGGACC11150.021.0717498
CAAGACG11300.020.6283174
GACGGAC11250.020.3911117
TTTAGCG555.1457743E-420.18181826
AGACGGA12050.019.190876
CGGACCA12300.019.1016279
TAACGGC1650.019.06060636
GCGCAAG12400.018.7983861
CGCAAGA12650.018.4268782
TCTAGCG5900.018.18644128
TTAACGG1750.017.97142835
CTAGCGG6150.017.44715529
TAGGTCG752.068679E-417.26666621
TACACCG1107.8180165E-716.8181825
GTATGGT13350.016.6292133
CGCAATA6650.016.41353436
ATCGCTC8850.016.30508416
CTATACG803.3848605E-416.18754
Produced by FastQC (version 0.11.5)