FastQCFastQC Report
Thu 28 Apr 2016
ERR1042120.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042120.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12695807
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT441470.3477289785517376No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT407970.32134231404116337No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC308310.24284395627627292No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT298710.23528240465533226No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG256760.20223999939507586No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA224610.1769166780811964No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC220860.17396294697926645No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA193930.1527512193592735No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT185410.14604034229568863No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG162820.12824706613766262No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG160520.12643544439514556No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG151790.11955915838985265No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA144470.11379347527888538No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC142510.11224965848961001No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC142020.11186370429229116No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138440.10904387566698201No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC128050.1008600713605681No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG38150.019.6880725
ACGGACC38400.018.9335948
GACGGAC38650.018.8589927
CAAGACG41050.018.4774674
TCGTTTA27700.017.8321330
CGGACCA41300.017.7384999
CGCAAGA43450.016.5627162
GCGCAAG44850.016.4994411
AGACGGA44450.016.481446
CTAGCGG22050.016.36054429
TCTAGCG22400.016.27008828
TTAACGG6050.015.90082635
TATACTG21750.015.7356325
TAAACGC23950.015.6033428
CGTTTAT32750.015.53435231
CGAGCCG34400.015.32703515
TAACGCC33600.015.3065474
GTAAACG24350.015.19507127
CCGGTCG33650.015.00891620
TAACGGC6550.014.96946636
Produced by FastQC (version 0.11.5)