FastQCFastQC Report
Thu 28 Apr 2016
ERR1042119.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042119.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4220982
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT316800.7505362496215336No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT289090.684888018949145No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT198480.47022233214924863No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123160.2917804435081694No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC68400.16204759935010382No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA68330.16188176116363442No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG54170.12833506515782347No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT52290.12388112529264517No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA46590.11037715868013652No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC46060.10912152669686817No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG44160.10462020449269861No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC44100.10447805747572485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG6450.019.21705428
AAGACGG10200.018.8627455
GGTATCA138000.018.660871
TAACGGC1900.018.536
CGAACTA2950.018.18644129
ACGGACC10600.017.8018888
GACGGAC10350.017.695657
CTAGCGG7100.017.45774729
CGCAATA7650.017.16993536
CAAGACG11400.016.8771934
GCGAACT3300.016.8181828
TCGTTTA9500.016.74736830
TAAACGC7550.016.66225228
GTAAACG7600.016.55263127
CGGACCA11500.016.4086959
GTATTAG11600.016.267241
TTAACGG2400.016.187535
TAGCGGC7950.015.82389830
CGTTTAT10350.015.55072431
TACCGTC9300.015.5161297
Produced by FastQC (version 0.11.5)