FastQCFastQC Report
Thu 28 Apr 2016
ERR1042118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3020849
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT223640.7403216777799884No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT218050.7218169461631482No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT129940.43014397608089644No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93860.3107073541246186No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC64080.21212579642345578No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA61210.20262515604057002No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG47620.15763780314739334No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC41010.13575653731782025No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT39650.13125449170084305No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA37910.12549452157323984No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC36860.12201867753072067No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC36800.12182005787114815No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG35440.11731801225417092No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG32360.10712220306278136No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93050.019.3648571
AAGACGG7150.016.559445
ACACGCT18850.016.1936349
TATAACG1151.2437577E-616.0869562
ACGCTGA19100.016.07853311
TAACGGC2650.016.05660436
GACGGTA6000.016.0333338
TACGACG6350.016.0236235
CGATAGA2100.015.85714237
TCGGTAA700.002593804415.85714227
TATACTG4450.015.7977525
GCGGGTA6850.015.66423423
ATAACGA7850.015.5541412
CACGCTG19850.015.28463510
CGTTCGG855.367742E-415.23529424
ACCCGTT9850.015.21319730
CGGACCA7300.015.205489
ATAGGCG2201.8189894E-1215.13636432
ACGGACC7350.015.102048
TAACGAA8100.015.07407413
Produced by FastQC (version 0.11.5)