FastQCFastQC Report
Thu 28 Apr 2016
ERR1042117.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042117.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3623353
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT161350.4453057706494509No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT148620.41017256668064084No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT105620.29149795783077165No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62380.17216097907104275No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC56690.1564572924581182No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA51740.14279591306726117No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG42460.11718427655268476No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT38610.10655875924868484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57150.023.9221341
TCGTTTA5700.018.50000230
TATAGCG500.007037733718.52
ACGGACC8300.017.3855428
AAGACGG8900.017.2528085
CGCATCG6350.016.89763813
GACGGAC8450.016.8579887
CGCAAGA9350.016.8181822
TAACGCC6450.016.3488374
GTATCAA84000.016.253572
TAACGGC2200.015.97727336
TACACCG2550.015.9607845
ATAACGC7200.015.9305553
AACGCCG6750.015.8962965
CGGACCA9650.015.7202069
TCGCCGG6950.015.70503617
CAAGACG10300.015.6262144
CGTTTAT6750.015.62222231
GCGCAAG9900.015.3232331
CCGGTCG6900.015.28260920
Produced by FastQC (version 0.11.5)