FastQCFastQC Report
Thu 28 Apr 2016
ERR1042115.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042115.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6310540
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT386970.6132121815248774No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT347850.551220656235441No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT234360.3713786775775132No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146270.23178682014534413No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT79730.12634417973739173No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA79200.12550431500315345No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC76030.1204809731021434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG15350.020.609125
ACGGACC15550.019.5112538
GACGGAC15750.019.4984137
TAACGGC2600.019.21153836
CAAGACG17150.018.9854224
CGCAAGA17000.018.9352952
CGGACCA16550.018.4441099
GCGCAAG17350.018.1268021
TTAACGG2900.017.8620735
GGTATCA146050.017.7083191
AGACGGA18950.016.303436
TCGTTTA10750.015.66046430
GTAAACG9850.015.40101627
TCTAGCG9150.015.16393528
TATACTG12050.014.8921165
CTAGCGG9500.014.60526329
GCAAGAC27050.014.4990763
CCGGTCG11550.014.41558520
GCGAAAG21300.014.33098618
TTCGGGC10600.014.13679235
Produced by FastQC (version 0.11.5)