FastQCFastQC Report
Thu 28 Apr 2016
ERR1042114.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042114.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2703418
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA61840.22874745969731652No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC61320.22682396876842575No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC46610.17241136960691983No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC44590.16493934715238265No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA42790.15828110932160694No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT41830.15473004914519323No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG39670.14674016374826238No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG39630.1465922029075785No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT38260.14152454411415474No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC31820.1177028487640461No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG31570.11677809350977171No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC30980.11459567110968412No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAG28550.1056070500381369No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGG28520.10549607940762398No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGAC8450.020.1420147
ATAATAC1202.401066E-1020.0416683
ACGGACC8750.019.2399988
AAGACGG9500.019.084215
CAAGACG9350.018.7967914
CCTAACG500.007037282518.514
AGACGGA9400.018.56
TCGTTTA6800.018.22794230
CTATACT2650.018.1509444
TAATACC1850.018.04
CGTTTAT7350.017.11564631
TCTATAC1851.8189894E-1217.03
CGGACCA10650.016.8497669
GCGCAAG10650.016.6760561
CTAGATA4900.016.6122463
CGCAAGA10400.016.3653852
CTATACA1606.311893E-1016.18754
TTAGGAC2300.016.0869563
GTTCAAA11050.016.07241
TAGACAG4150.016.0481935
Produced by FastQC (version 0.11.5)