FastQCFastQC Report
Thu 28 Apr 2016
ERR1042113.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042113.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3348327
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT282610.8440334531245007No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT205750.6144859806106154No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT127470.3806975841965256No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81620.24376352727795103No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT57840.17274298478016037No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA39420.11773043672257819No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG36930.11029388706658579No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA36010.10754624622983358No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC35830.10700866432699077No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT35350.10557511258607657No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGACG12350.024.4170044
ACGGACC12300.023.6138238
AAGACGG13150.023.0722435
GACGGAC12550.022.9960167
CGGACCA13250.022.1999999
AGACGGA13450.021.8698886
CGCAAGA14050.020.9359442
GCGCAAG14900.020.1140941
GCAAGAC15750.019.4984133
TCGTTTA7600.018.74342230
GCGAAAG15800.018.03164518
GAGCGAA15800.018.03164516
CGAAAGC15850.017.97476219
AGCGAAA16000.017.8062517
CAGAGCG16550.017.43806614
CGTTTAT8350.017.28143731
AGAGCGA16800.017.17857215
CGAGCCG10450.017.1722515
TCTAGCG5300.017.10377328
TAACGCC9100.016.2637354
Produced by FastQC (version 0.11.5)