FastQCFastQC Report
Thu 28 Apr 2016
ERR1042112.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042112.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1730827
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT390412.255626934407656No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT278121.6068619220754008No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133730.7726364333350474No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT100600.5812250444440721No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT50990.29459905582706997No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA34490.19926890440234638No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC31820.18384275262634567No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT31080.17956733977457018No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG27480.15876803400917597No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA24930.14403519242535506No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG24410.14103084825924256No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA24170.13964422787488293No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23040.13311555689852309No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21230.12265812816647764No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG20310.11734275002643245No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20200.11670721568360096No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGG19610.11329844057205024No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19260.11127628584485913No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC19160.11069852735137596No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA18500.10688532129438702No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG18450.10659644204764544No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA17680.102147701647825No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG8750.023.891435
CAAGACG9750.022.5794874
GACGGAC9650.021.0880837
ACGGACC9700.020.9793828
GCGCAAG9750.020.8717941
CGCAAGA9750.020.8717942
CGGACCA9850.020.8477159
AGACGTA450.003826578420.5555555
ACGTAAG450.003826578420.5555551
TAGACCG555.1445386E-420.18181825
CTAGTAC555.1445386E-420.1818183
AGACGGA10300.019.757286
TCACGTA1052.2613676E-819.3809525
TCTATAC1153.0540832E-919.3043483
GCAAGAC12500.018.9443
TTAATCG1103.8549842E-818.537
ATAGACG801.6174412E-518.53
GCCGCGA701.21964964E-418.532
CGCATTC500.00703628618.49999812
TGTACGA500.00703628618.4999982
Produced by FastQC (version 0.11.5)