FastQCFastQC Report
Thu 28 Apr 2016
ERR1042111.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042111.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1153264
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT93720.8126500090178832No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT92820.8048460716713606No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT46470.4029432983254484No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39230.3401649578934225No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23300.20203526685997308No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG18470.16015413643363532No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG16720.144979813815397No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC16400.14220508053663342No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCG15370.13327390779561316No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA14850.12876496621762232No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG11880.10301197297409787No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA11570.10032395011029564No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGACG8750.024.5257154
AAGACGG8850.024.0395475
CGGACCA9300.022.4784959
GCGCAAG9500.022.0052621
ACGGACC9500.022.0052628
GACGGAC9500.022.0052627
CGCAAGA9700.021.932992
AGACGGA9800.021.5204096
GATCACG450.00382581620.55555531
GCAAGAC11300.019.6460173
GGTATCA34000.019.5882341
TTATCTA2000.019.4254
TCGCGTA7050.019.1560299
GTCGCGT7150.019.1468528
GCGGGTA1457.2759576E-1219.13793223
GGACCAG11050.019.08597410
CGAAAGC10950.018.92237519
AGCGAAA11000.018.83636517
CGCGTAA7300.018.75342410
GAGCGAA11400.018.66228116
Produced by FastQC (version 0.11.5)