FastQCFastQC Report
Thu 28 Apr 2016
ERR1042110.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042110.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4584340
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT397100.8662097488406182No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT365940.7982392230942732No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT237780.5186788065457624No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214820.46859526125898165No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT62450.13622462557314685No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA56210.12261306971123433No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC54590.11907930040093012No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA53220.1160908658607346No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG49930.10891426028610443No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47050.10263200373445251No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG46680.10182490827469166No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT46670.10180309488388732No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG11900.020.8319345
GACGGAC11950.019.81597
CAAGACG14100.019.2872334
TCTAGCG7550.018.8675528
ACGGACC12750.018.7176488
CTAGCGG7700.018.529
CGCAATA8250.017.71515336
CGCAAGA14000.017.5752
GCGCAAG15100.017.5198691
CGGACCA14000.017.1785729
CGGTCCA8650.016.6820810
AGACGGA14750.016.5559336
GCAAGAC19250.016.0493493
GCGGGTA8450.015.32544323
GTAAACG8050.015.16770227
TAACGGC2950.015.05084736
TTAACGG3100.014.91935535
GTATTAG15900.014.8930811
CCGGTCC9950.014.8743729
CGAGCCG11250.014.815
Produced by FastQC (version 0.11.5)