FastQCFastQC Report
Thu 28 Apr 2016
ERR1042109.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042109.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2393972
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT354441.480551986405856No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT259621.0844738367867293No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT163180.6816286907282124No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144110.6019702820250195No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA46600.1946555765898682No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC41480.17326852611475824No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT36870.1540118263705674No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG36640.15305107996250583No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT31510.13162225790443663No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28100.11737814811534973No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA26540.11086178117371463No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA26230.10956686210197948No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG25470.10639222179708034No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24980.10434541423207956No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24800.10359352573881399No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC24290.10146317500789484No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAA358.872211E-426.4285721
CAAGACG8050.020.9130444
AAGACGG8150.020.2024565
GACGGAC7900.020.139247
GCGCAAG8200.019.4024371
ACGGACC8250.019.284858
CGGACCA8500.018.2823549
AGACGGA8800.018.0795466
GCAAGAC11000.017.9954533
CGTGCGA1351.1532393E-917.81481632
CGCAAGA9250.017.82
ATTGACG7250.017.09655232
ACACGCT7000.016.9142869
CGATCGG1352.2226232E-816.44444536
GTATGGT8000.016.418753
TTGTACG803.3844786E-416.18754
GTATTAG5500.016.1454541
ACGCTGA7400.016.011
GTATAGG1851.8189894E-1116.01
ATAATAC3150.015.8571423
Produced by FastQC (version 0.11.5)