FastQCFastQC Report
Thu 28 Apr 2016
ERR1042108.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042108.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4803035
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT163120.3396185953256639No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT152890.31831956252661076No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT103970.21646729619917404No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT94390.19652157437953294No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74600.1553184600986668No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG60760.12650334632164872No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT59780.12446296976807374No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA56720.11809199808038041No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC54660.11380304328409017No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC54250.11294941635861491No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA50430.1049961118334553No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG48630.10124848142892982No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA48420.1008112578817352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG18050.020.2936295
GACGGAC17900.019.5335187
CAAGACG18600.019.395164
ACGGACC18300.019.3087448
CGGACCA18750.018.8453339
GGTATCA68500.018.2029191
AGACGGA20650.018.1864416
CGCAAGA20850.017.8345322
GCGCAAG21600.017.043981
GCAAGAC25600.016.0429693
TCTAGCG9600.015.99479128
TATACTG8950.015.91625
GTAAACG10350.015.90821227
TCGTTTA10850.015.68663630
CTAGCGG9850.015.58883329
TAAACGC10850.015.34562228
CGAACGA7050.015.21985816
ACGCTTC11250.014.96444531
TAACGCC12600.014.8293664
GCGTTAT11750.014.81
Produced by FastQC (version 0.11.5)