FastQCFastQC Report
Thu 28 Apr 2016
ERR1042107.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042107.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386126
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT41881.084620046306128No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT32540.8427300932856114No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21990.5695032191564412No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT16990.44001180961655006No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13210.34211630400439236No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5270.13648394565504524No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCG5220.13518903155964634No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG4810.12457073597737527No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA4290.11110362938522658No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4130.10695990427995007No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCC251.2321075E-437.036
AACCGAG303.5950917E-430.8333327
CGGTTAA250.00549187929.61
CCGTAGG405.9300717E-527.7500022
GGTATCA9550.027.5078531
CCCGTAG358.858687E-426.428571
AGGTTAT509.0706835E-625.9000021
CGGACCA3300.023.5454549
GACGGAC3250.023.338467
CAAGACG3500.023.2571434
TAGCAGT400.001928877623.1250024
TCTATAC400.001928877623.1250023
AAGTCGA400.001928877623.12500223
ACGGACC3300.022.9848488
GTATCAA11500.022.8434772
CGAAAGC3300.022.42424219
AGACGGA3400.022.3088256
TATAGTG603.7180427E-521.5833325
TTGCCAA3450.021.44927628
GCGAAAG3500.021.14285918
Produced by FastQC (version 0.11.5)