FastQCFastQC Report
Thu 28 Apr 2016
ERR1042106.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042106.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4930762
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT176680.35832189831916444No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT175550.35603016328916304No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT123230.2499208033159986No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT75960.15405326803443362No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70310.14259459288442639No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA69710.14137774242601853No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC66310.13448225649504073No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG56540.11466787486396626No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG54800.11113900853458351No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC53440.10838081416219238No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA50250.1019112258916573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68100.021.2165931
AAGACGG15100.020.3377485
GACGGAC15450.019.3980587
ACGGACC16250.018.898468
CAAGACG17050.018.3372444
GCGCAAG17150.018.1224481
CGCAAGA17050.017.9032272
CGGACCA17250.017.6956529
GTAAACG9400.017.51595927
AGACGGA17500.017.3371436
CGAACGA9200.017.29347816
TAAACGC9900.017.0050528
ACGAACG9450.016.8359815
TTCGGGC9850.016.71573635
ATAACGA9800.016.61224412
TAACGAA9800.016.4234713
TCCGATA10300.015.9854368
CTTCGGG11000.015.80909134
TCTAGCG6450.015.77519428
CGCTTCG10600.015.70754732
Produced by FastQC (version 0.11.5)