FastQCFastQC Report
Thu 28 Apr 2016
ERR1042105.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042105.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2663144
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT143900.5403387875383381No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT125230.47023367868954885No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT77800.2921359115391432No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53510.20092792578996857No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT44890.1685601679819041No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC33660.12639196378415887No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG31830.11952038643047466No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA31770.11929508881232108No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC29140.10941954321658913No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA26820.10070803531465064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGACG9150.020.2185784
GACGGAC8900.019.9550557
AAGACGG9450.019.7724885
ACGGACC8900.019.747198
TATACCG1052.2628228E-819.380955
GGTATCA52350.018.3763141
TCGTTTA5850.018.3418830
CGGACCA9700.017.7371149
GCGCAAG10300.017.601941
CGCAAGA10200.017.5931382
AGACGGA10300.017.4223296
GTAAACG4500.017.26666627
CGTTTAT6850.015.93430731
TTCGGGC4550.015.85714335
TGCGTAT700.002593696115.85714210
TACCGTC6550.015.8167947
CGCATCG7050.015.7446813
CCGGTCG6700.015.73880620
CGGGTAA3800.015.57894824
GCGAAAG11350.015.48458118
Produced by FastQC (version 0.11.5)