FastQCFastQC Report
Thu 28 Apr 2016
ERR1042104.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042104.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5355524
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT167240.31227569888586065No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT157450.29399550818930137No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT107620.20095139149782543No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT91310.1704968552096863No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC72970.1362518401560706No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA70640.13190119211490792No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT64320.12010029270711886No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG62980.11759820327572054No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58850.10988653958044069No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC58070.10843009946365659No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG55250.10316450827220641No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA54810.10234292666786668No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG17550.020.2393175
GACGGAC18300.019.1065587
ACGGACC18450.018.951228
TCTAGCG9600.018.11458228
CAAGACG19750.018.078484
CTAGCGG9650.018.02072529
CGGACCA19600.017.9336729
CGCAAGA19700.017.8426382
TCGTTTA12450.017.83132630
AGACGGA20000.017.66756
GGTATCA75450.017.5314791
CGTTTAT13300.017.2481231
GCGCAAG20850.016.8585131
CCGGTCG14600.015.96575420
TAGCGGC11100.015.66666730
AACGCCG15300.015.5980395
TAACGCC14950.015.4682284
TAAACGC10900.015.44495428
TACGACG13100.015.393135
ATCGTTT15050.015.24252629
Produced by FastQC (version 0.11.5)