FastQCFastQC Report
Thu 28 Apr 2016
ERR1042103.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042103.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1910864
Sequences flagged as poor quality0
Sequence length43
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC45070.23586189284009748No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA36790.19253070862185903No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC36270.1898094265211967No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC26830.14040768992455768No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC22570.11811410963836254No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC20520.10738597827998225No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT20160.10550201374875448No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG19120.10005944954742986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTA3200.023.12530
TATAACG400.001931653223.1252
TATACTG1700.020.6764725
CGTTTAT3750.019.73333231
CCTATAC1551.8189894E-1219.0967733
GTTCAAA6950.018.6330951
CTATAGA801.6175845E-518.52
GTACTAG609.239867E-418.51
TACCGCA5750.018.33912828
GGTATCA9600.018.1145841
ATGGTCG4050.017.81481636
TATGGTC4050.017.35802535
ATAACGA2850.016.87719312
TAACGAA2750.016.81818213
ATACCGC6300.016.73809627
ATTACTA904.4484375E-516.4444455
GTATATA904.4484375E-516.4444452
ATCGTTT4850.016.02061829
TTAGGAC1403.4768163E-815.8571433
TAGATAG700.00259333615.8571435
Produced by FastQC (version 0.11.5)