FastQCFastQC Report
Thu 28 Apr 2016
ERR1042102.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042102.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7858956
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT288660.3673006949014602No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT263290.335019053421345No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT173490.22075451243142222No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98640.12551285437913126No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT94770.1205885361872493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG21700.019.4377885
ACGGACC20650.018.4552048
GACGGAC20300.018.4088677
TCGTTTA13200.017.6590930
GGTATCA124150.016.6596851
CGCAAGA22650.016.4988962
AGACGGA24200.016.2830586
CGGACCA24100.016.1970969
GCGCAAG24950.016.164331
TAACGCC16150.016.0371514
CAAGACG26200.015.9580154
TCTATAC12550.015.920323
CGTTTAT15300.015.35620931
ACGAACG8950.015.29608815
ATAACGC17300.015.2919073
TCTAGCG11500.015.28260928
CTAGCGG11750.015.27234129
TATACCG3650.015.205485
AATAACG16250.015.1415392
CGAACGA9050.015.12707316
Produced by FastQC (version 0.11.5)