FastQCFastQC Report
Thu 28 Apr 2016
ERR1042101.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042101.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8354724
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT187880.22487876320031636No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT183450.21957637379762635No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT125420.15011866340527827No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT102480.12266114356380893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78250.019.812141
ACGGACC22450.018.3763928
GACGGAC22200.018.257
AAGACGG24400.018.045085
TCGTTTA12350.016.77732830
CAAGACG27100.016.7250924
CGGACCA24900.016.7168679
CGCAAGA25500.016.5411762
TATACCG3950.016.3924055
AGACGGA25600.016.18756
CTAGCGG11150.015.92825129
TCTAGCG11550.015.53679728
TACGACG16250.015.4830775
TATACTG14850.015.3232325
GCGCAAG27050.015.1829941
CGAGCCG20800.015.0312515
GTAAACG13250.014.79999927
TAAACGC13650.014.637362528
GTATTAG26800.014.2201491
ACGACGG17350.014.1815576
Produced by FastQC (version 0.11.5)