FastQCFastQC Report
Thu 28 Apr 2016
ERR1042100.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042100.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6303438
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT107130.16995487224590772No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG72290.11468344735047761No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC67890.10770312962545203No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA67200.10660848889130028No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39000.021.4410251
ACGGACC22850.019.5929978
CAAGACG23950.019.388314
GACGGAC23150.019.259187
AAGACGG23700.019.2025325
CGGACCA25000.018.139
CGCAAGA25650.017.8148142
GCGCAAG25500.017.774511
AGACGGA25800.017.4961246
TCTAGCG8850.016.51412628
TACGACG14850.016.4444455
GTATACG1951.8189894E-1216.1282061
TACCGTC16250.016.0523077
GCAAGAC32350.016.0123653
TATACTG10450.015.9330165
ACGAACG9450.015.85714315
CGTCGTA14900.015.76845710
GTATCAA52850.015.6821192
TAACGAA9700.015.63917513
CGAAAGC28050.015.63101719
Produced by FastQC (version 0.11.5)