FastQCFastQC Report
Thu 28 Apr 2016
ERR1042099.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042099.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5969190
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT285030.4775019726294522No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT240530.4029524943920364No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT161670.2708407673402924No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90780.15208093560432823No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT87550.14666981617271355No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA64930.10877522745967208No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC61640.10326359187762493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG17100.019.4736845
ACGGACC17450.018.7650418
CGCAAGA18150.018.5509662
GACGGAC17750.018.0309857
CAAGACG19750.017.984814
GCGCAAG19700.017.185281
GGTATCA113300.016.5732571
CTAGCGG11500.016.56956529
TCTAGCG11550.016.49783528
CGGACCA19950.016.4135349
TCGTTTA11850.016.39240530
AGACGGA19950.016.228076
GTAAACG10500.015.68095327
CGGTCCA12400.015.36693510
TAACGCC13950.015.2508964
CGCAATA13300.015.161653536
TATCTAG15650.015.012781
GCAAGAC27800.014.7068353
TCTATAC7300.014.6986293
TAGAGTC18050.014.6565095
Produced by FastQC (version 0.11.5)