FastQCFastQC Report
Thu 28 Apr 2016
ERR1042098.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042098.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4497543
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT211440.4701233540179605No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT197000.43801693502430106No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT114930.25553952458042095No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69180.1538173175887368No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT57550.12795875436877424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGAC12600.020.4087317
ACGGACC12650.020.3280648
AAGACGG13400.020.0186565
GGTATCA77200.019.5304411
CGGACCA13500.018.9111129
CGCAAGA14350.018.564462
CAAGACG14650.018.436864
AGACGGA14700.018.2482996
TCGTTTA7250.017.60689730
GCAAGAC19950.016.5062663
GCGCAAG16250.016.3938471
CGAACGA5100.016.3235316
GTAAACG7350.015.85714227
GAGCGAA16400.015.67987716
CGTCGTA7350.015.60544210
TAAACGC7400.015.528
TACGACG8850.015.4689265
CGAAAGC16700.015.28742519
ACGAACG5450.015.2752315
GTATTAG11950.015.1715491
Produced by FastQC (version 0.11.5)