FastQCFastQC Report
Thu 28 Apr 2016
ERR1042097.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042097.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7473879
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT342080.4577007468277183No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT327290.43791182597416944No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT207770.2779948671901164No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143630.19217597715991924No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT85380.11423786764543553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC18300.020.5218568
GACGGAC18450.020.2547447
AAGACGG20100.019.8805985
CGGACCA19900.018.314079
AGACGGA20800.018.144236
CGCAAGA21050.017.8408572
GGTATCA143100.017.4140471
TAAACGC11700.016.44444528
GTAAACG11600.016.42672327
TACCGTC13400.016.2910447
CTAGCGG9200.016.28804429
TCTAGCG9100.016.26373528
CAAGACG24650.016.2109554
TCTATAC11300.016.2079643
TTCGGGC12500.016.13235
GCGAAAG23400.015.57478518
TAAGACG2500.015.544
GCGCAAG24400.015.4672141
GTATTAG22850.015.4638961
CGAAAGC24250.015.10515419
Produced by FastQC (version 0.11.5)