FastQCFastQC Report
Thu 28 Apr 2016
ERR1042096.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042096.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6717233
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT109580.163132647028918No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT95530.1422162965018483No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT73700.10971779600320548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46500.021.4043031
TAACGGC4500.017.26666636
GACGGAC14700.016.2346947
TTAACGG4800.016.187535
ACGGACC14950.015.8394668
AAGACGG16950.015.8259575
TACGACG11450.015.6724885
ACGACGG11200.015.0312496
CGCAAGA16800.014.7559522
TAGACAG11950.014.7071135
GTATCAA68050.014.59882452
ACGAACG5350.014.52336415
GTATTAG21950.014.4965831
GTCGTAC2059.276846E-1114.4390251
CGGACCA16500.014.3515149
AGACGGA17150.014.3469396
TCTAGCG6850.014.31386928
TAAACGC11600.014.19396528
GTAAACG11600.014.19396527
TACCGTC8100.013.9320987
Produced by FastQC (version 0.11.5)