FastQCFastQC Report
Thu 28 Apr 2016
ERR1042095.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042095.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4438617
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT106820.24066054809414733No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT101770.2292831303083821No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT67790.15272775281129233No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT66190.1491230263841192No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT45120.10165328524628278No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG44490.10023392421558337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44550.022.2996641
GACGGAC13000.019.6384627
ACGGACC13150.019.2737648
AAGACGG13700.019.0401485
CGGACCA14500.017.8620689
CGCAAGA14400.017.857642
AGACGGA14800.017.756
TTAACGG3400.017.41176435
CAAGACG15300.017.4117644
TAACGGC3450.017.1594236
GCGCAAG16050.016.7133941
GTATTAG15300.016.4444451
GTATCAA62050.015.9508452
CGGGTAA5300.015.35849124
TATACTG8100.015.3024695
GCGGGTA5200.015.29807723
ACCCGTT7100.015.11267730
TCTAGCG6400.015.03125128
CGAGCCG14450.014.979238515
TACCGTC7800.014.9423087
Produced by FastQC (version 0.11.5)