FastQCFastQC Report
Thu 28 Apr 2016
ERR1042094.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042094.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9653584
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT399220.41354589134978265No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT392980.40708197079965325No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT265000.2745094464397886No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180270.18673893550830448No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT108340.11222774878221393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA153400.018.837681
GACGGAC22800.018.337727
ACGGACC23800.017.8004198
AAGACGG24850.017.7927555
CGGACCA24800.017.0826639
CGCAAGA26750.016.4598142
GCGCAAG28100.015.93238451
TCGTTTA14800.015.7530
CAAGACG28900.015.4913494
TCTAGCG11500.015.44347828
AGACGGA28150.015.378336
GTAAACG16350.015.2752327
CGAACGA10700.015.21495216
TACCGTC16000.014.87
ACGAACG11150.014.76681715
CGAGCCG20150.014.41439215
ACGCTTC18150.014.37190131
GCGGGTA13150.014.3498123
TAAACGC17550.014.33618328
CGGGTAA13300.014.32706824
Produced by FastQC (version 0.11.5)