FastQCFastQC Report
Thu 28 Apr 2016
ERR1042093.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042093.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1627213
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT69170.4250826413014153No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT61250.3764104637807097No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT39350.24182451836360697No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25150.15455874553607918No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT24400.1499496378163154No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG17450.10723857294650423No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACG451.3235425E-424.66666622
GGTATCA26700.021.6179771
CGCAAGA5400.019.1851842
GACGGAC5100.018.8627457
AAGACGG5450.018.3302765
ACGGACC5350.018.3271038
GTATTAG5700.018.1754381
CGGACCA5500.018.1636379
CGAACGA1950.018.02564216
GCGCAAG5900.017.872881
CTTCGGG3150.017.6190534
TTTAGCG1054.800295E-717.61904726
GACGCTA650.001580315417.07692310
CGTGATA1201.0419535E-716.95833224
GACGGTA4400.016.8181828
TATACTG2750.016.8181825
AGACGGA5850.016.7606856
TACGACG4550.016.670335
GTAAACG3250.016.50769227
CGACACG904.447784E-516.44444536
Produced by FastQC (version 0.11.5)