FastQCFastQC Report
Thu 28 Apr 2016
ERR1042092.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042092.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8215486
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT258740.3149418062425035No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT249230.30336610639954836No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT169750.20662198194969839No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94510.11503884249817965No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT86300.10504552013112797No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84700.023.6109811
GACGGAC18900.018.0105827
ACGGACC19550.017.4117648
AAGACGG22000.016.8181825
CGCAAGA21650.016.3210162
GTATCAA123550.016.0967222
TCTAGCG10950.016.0502328
AGACGGA22500.016.0333336
CGGACCA22100.015.6538479
GCGCAAG24200.015.5950421
TACGACG13800.015.4166675
CAAGACG24750.015.3232324
TAAACGC16500.015.24848428
GTAAACG16700.014.9550927
CTAGCGG11850.014.83122429
TACCGTC11950.014.7071137
ACGACGG14450.014.4671296
ATACCGT14850.014.3265996
CGACGGT14500.014.2896557
CGAACGA8050.014.24844716
Produced by FastQC (version 0.11.5)