FastQCFastQC Report
Thu 28 Apr 2016
ERR1042091.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042091.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9170568
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT226870.24738925658694205No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT201280.21948476910045267No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT148100.1614949041324376No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT100590.10968786230035042No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81100.023.3360061
ACGGACC22000.020.0977298
GACGGAC22200.019.5833347
AAGACGG25600.018.0664065
AGACGGA26200.017.5114526
CGGACCA25300.017.2569169
CAAGACG27650.016.860764
CGAACGA9250.016.616
CGCAAGA26200.016.2404582
GCGAAAG26450.016.08695618
GTATCAA119650.015.8173852
CGAAAGC27900.015.51612919
ACGAACG9900.015.51010115
GAGCGAA28300.015.2968216
AGCGAAA28250.015.25840817
GTATTAG23600.015.0508471
CGAGCCG22250.014.80000115
TATACTG17550.014.6524225
TCGTTTA11650.014.60944230
GCGCAAG30250.014.4330591
Produced by FastQC (version 0.11.5)