FastQCFastQC Report
Thu 28 Apr 2016
ERR1042090.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042090.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10968359
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT353070.32189865411954516No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT316740.2887761058878543No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT221830.20224538602356107No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT134710.12281691363311505No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127780.11649873969296592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124100.022.346091
AAGACGG30850.018.2301455
ACGGACC30050.018.1613988
GACGGAC29900.018.1287617
CAAGACG35000.017.2314284
CGGACCA32350.016.870179
CGCAAGA32750.016.6076352
TCTAGCG14200.016.41549328
CTAGCGG14600.016.2191829
GTATCAA175950.015.7189542
GCGCAAG35600.015.6938191
GTAAACG20800.015.56490427
AGACGGA36200.015.2292816
TATACTG20000.014.9855
CGAAAGC36600.014.60792419
GCGAAAG36800.014.57880318
TAAACGC22400.014.45312428
GTATTAG28150.014.2611021
GAGCGAA37750.014.21192116
CGAGCCG27600.014.14311515
Produced by FastQC (version 0.11.5)